Serological grouping of streptococci by a slide coagglutination method.

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Serological grouping of streptococci by a slide coagglutination method.

A new method for the serological grouping of streptococci by coagglutination with specific antibodies absorbed to protein A-containing staphylococci has been assessed. A total of 242 strains of streptococci, including beta-haemolytic streptococci of groups A, B, C, F, and G, Streptococcus pneumoniae and Strep. faecalis were studied. All streptococci of groups A, B, C, and G, groupable by standa...

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Serological grouping of streptococci by slide agglutination.

Streptococcal grouping sera for groups A, B, C, and G prepared for conventional testing by precipitation were made specific by absorption and used to identify streptococci by slide agglutination with and without staphyloccocal coagglutination. Trypsinised suspensions of 1055 strains, identified by precipitation as belonging to group A, B, C, or G, were tested by slide agglutination. Of these, 9...

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Simplified coagglutination test for serological grouping of beta-hemolytic streptococci.

A new coagglutination kit consisting of a plastic slide with dried antibody-coated staphylococci was evaluated for the grouping of beta-hemolytic streptococci of groups A, B, C, and G. The test was compared with the classical precipitation test, using hot formamide antigen extracts with 224 strains of groups A, B, C, and G streptococci. An agreement of 100% was found between the new coagglutina...

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Modified coagglutination procedure for the serological grouping of streptococci.

Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the supernatant was tested by slide coagglutination. Positive coagglutination rea...

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Bacitracin and coagglutination for grouping of beta-hemolytic streptococci.

Bacitracin may be used for presumptive differentiation of group A from other beta-hemolytic streptococci. Whatever criteria are used for the test, a small number of erroneous results will be obtained. With the 1,161 strains studied in this laboratory, using a zone of 10 mm or greater as indicative of group A was better than using Maxted's original criterion of any zone of inhibition for group A...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1977

ISSN: 0021-9746

DOI: 10.1136/jcp.30.2.168